master's thesis
Identification of Vrn genes in Croatian wheat varieties

Josipa Puškarić (2015)
Metadata
TitleIdentifikacija Vrn gena u hrvatskom sortimentu ozime pšenice
AuthorJosipa Puškarić
Mentor(s)Sonja Petrović (thesis advisor)
Abstract
Niske temperature u odreĊenim fazama razvoja ţitarica su potrebne kako bi došlo do klasanja i formiranja zrna, što izravno utjeĉe i na prinos. Cilj ovog istraţivanja bio je ispitati genetsku varijabilnost Vrn-A1, Vrn-B1, Vrn-B3 te Vrn-D1 genlokusa u 23 hrvatska ozima i 17 stranih kultivara korištenjem molekularnih markera. Gen Vrn-A1 se sastoji od ĉetiri razliĉite alelne varijante. Vrn-A1a alel pronaĊen je u jednom hrvatskom kultivaru (4,35%), te u dva strana (11,76%). U šest hrvatskih (26,10%) kultivara utvrĊen je Vrn-A1b (ili Vrn-A1c) alel, dok kod stranih nije pronaĊen ni u jednom kultivaru. Recesivni vrn-A1 alel pronaĊen je u 16 kultivara (69,6%) hrvatskog podrijetla te u svim stranim kultivarima. Na Vrn-B1 lokusu pronaĊen je recesivni vrn-B1 alel u 20 hrvatskih (87%) i 16 (94,08%) stranih kultivara, dok je recesivni vrn-B3 alel pronaĊen u ĉak 22 hrvatska kultivara (95,7%), te u svih 17 stranih. Dominantni Vrn-D1 alel nije pronaĊen u hrvatskim kultivarima, a u stranim pronaĊen je samo u kultivaru Chinese Spring (5,88%). U svim hrvatskim kultivarima identificiran je recesivni vrn-D1 alel te u 14 stranih kultivara (82,32%).
Keywordswheat vernalization Vrn genes molecular marker
Parallel title (English)Identification of Vrn genes in Croatian wheat varieties
Committee MembersSonja Marić
Andrijana Rebekić
GranterSveučilište Josipa Jurja Strossmayera u Osijeku
Poljoprivredni fakultet
Lower level organizational unitsZavod za Bilinogojstvo
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Agronomy
Genetics and Breeding of Plants, Animals and Microorganisms
Study programme typeuniversity
Study levelgraduate
Study programmePlant Production, specialization: Plant Production, Plant Nutrition and Soil Science, Plant Selection and Seed Production, Plant Protection
Academic title abbreviationmag. ing. agr.
Genremaster's thesis
Language Croatian
Defense date2015-09-18
Parallel abstract (English)
Low temperatures during different developmental stages of cereals are needed in order for them to emergence and form kernel, thus they have a direct influence on a yield. The aim of this study was to examine genetic variability of Vrn-A1, Vrn-B1, Vrn-B3 and Vrn-D1 loci at 23 Croatian winter and 17 foreign wheat varieties using molecular markers. The Vrn-A1 gene, was identified with four different combinations of alleles. The Vrn-A1a allele was found in one Croatian wheat variety (4.35%) and in two foreign wheat varieties (11.76%). In six Croatian wheat varieties (26.10%) Vrn-A1b (or Vrn-A1c) allele was identified, while in terms of foreign wheat varieties there were no latter alleles. A recessive vrn-A1 allele was found in 16 wheat varieties of Croatian origin (69.6%) and in all foreign varieties. A recessive vrn-B1 was found on the Vrn-B1 locus in 20 Croatian (87%) and 16 foreign (94.08%) varieties, while a recessive vrn-B3 on the latter was found in 22 Croatian wheat varieties (95.7%), and in all foreign varieties. The dominant Vrn-D1 allele was not found in any of Croatian wheat varieties, while in terms of foreign varieties it was found only in Chinese Spring variety (5.88%). A recessive vrn-D1 allele was identified in all Croatian wheat varieties, while it was found only in 14 foreign varieties (82.32%).
Parallel keywords (Croatian)pšenica vernalizacija Vrn geni molekularni markeri
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
Notediplomski rad
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:151:232497
CommitterJosipa Vrbanić